Whole genome sequencing of V. fischeri strain derived from ES114 - SRA

SRX23390112: Whole genome sequencing of V. fischeri strain derived from ES114
1 ILLUMINA (NextSeq 500) run: 3.5M spots, 1G bases, 438.2Mb downloads

Design: Genomic DNA from V. fischeri strains was extracted using the Invitrogen PureLink Genomic DNA Mini Kit. DNA was sonicated to generate fragments of approximately 500 bp, and gDNA libraries were prepared using the NEBNext Ultra II DNA library prep kit for Illumina, which includes end-repair, adaptor ligation, and addition of index primers. Sequencing was performed by the University of Georgia Genomics and Bioinformatics Coreon an Illumina NextSeq500 instrument.

Submitted by: University of Georgia

Study: Vibrio fischeri D-glutamate suppressor generation and characterization

PRJNA1069005 • SRP485907 • All experiments • All runs

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This study used D-glutamate auxotrophic mutants of Vibrio fischeri to study the evolution of peptidoglycan biosynthesis and structure. Suppressor mutants were generated by growing an auxotrophic strain without necessary exogenous D-glutamate. Mutants were then characterized and genome sequenced, in order to determine the mechanism of suppression.

Sample:

SAMN39609067 • SRS20250972 • All experiments • All runs

Organism: Aliivibrio fischeri

Library:

Name: RMJ13S4

Instrument: NextSeq 500

Strategy: WGS

Source: GENOMIC

Selection: PCR

Layout: PAIRED

Runs: 1 run, 3.5M spots, 1G bases, 438.2Mb

Run	# of Spots	# of Bases	Size	Published
SRR27724179	3,459,245	1G	438.2Mb	2024-02-01

ID:: 31610665

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